Siglec-E Recombinant antibody

• 

  WB analysis of mouse spleen using 84790-2-RR

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84790-2-RR (Siglec-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Siglec-E Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84790-2-RR

Clone No.242248H3

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Sialic acid-binding Ig-like lectin 5, Sialic acid-binding Ig-like lectin 12, Myeloid inhibitory siglec, mSiglec-E, MIS

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Tested Applications

• Positive WB detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84790-2-RR targets Siglec-E in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: sialic acid binding Ig-like lectin E
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 70-90 kDa
• GenBank Accession Number: NM_031181.2
• Gene Symbol: Siglec-E
• Gene ID (NCBI): 83382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q91Y57
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily that contain sialoside-binding N-terminal domains (PMID: PMID: 9498912; 22749793). Mouse Siglec‑E, also known as myeloid inhibitory siglec (MIS), is a mouse CD33-related siglec that contains three extracellular immunoglobulin-like domains, a transmembrane region and a cytoplasmic tail bearing two immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which can recruit SHP-1 and SHP-2, two inhibitory regulators of immunoreceptor signal transduction (PMID: 11171044). Siglec-E is an inhibitory receptor and is expressed broadly on cells of innate immune cells including neutrophils, monocytes, macrophages, dendritic cells, and a subset of natural killer cells (PMID: 15048729; 31546700).