Mouse Siglec-E Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis of mouse spleen using 84790-2-RR (same clone as 84790-2-PBS)

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84790-2-RR (Siglec-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84790-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84790-2-RR (same clone as 84790-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84790-2-RR (Siglec-E antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84790-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84790-2-RR (same clone as 84790-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84790-2-RR (Siglec-E antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84790-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01535-1

  Cytometric bead array standard curve of MP01535-1, MOUSE Siglec-12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84790-2-PBS. Detection antibody: 84790-1-PBS. Standard: Eg2733. Range: 3.125-100 ng/mL.

Siglec-E Uni-rAb^® Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 84790-2-PBS

Clone No.242248H3

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

MIS, mSiglec-E, Myeloid inhibitory siglec, Sialic acid-binding Ig-like lectin 12, Sialic acid-binding Ig-like lectin 5

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Product Information

84790-2-PBS targets Siglec-E as part of a matched antibody pair:

MP01535-1: 84790-2-PBS capture and 84790-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2733

  Product name: Recombinant Mouse Siglec-12 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 19-350 aa of NM_031181.2

  Sequence: QNPQEVFTLNVERKVVVQEGLCVLVPCNFSYLKKRLTDWTDSDPVHGFWYREGTDRRKDSIVATNNPIRKAVKETRNRFFLLGDPWRNDCSLNIREIRKKDAGLYFFRLERGKTKYNYMWDKMTLVVTALTNTPQILLPETLEAGHPSNLTCSVPWDCGWTAPPIFSWTGTSVSFLSTNTTGSSVLTITPQPQDHGTNLTCQVTLPGTNVSTRMTIRLNVSYAPKNLTVTIYQGADSVSTILKNGSSLPISEGQSLRLICSTDSYPPANLSWSWDNLTLCPSKLSKPGLLELFPVHLKHGGVYTCQAQHALGSQHISLSLSPQSSATLSEMM

• Full Name: sialic acid binding Ig-like lectin E
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 70-90 kDa
• GenBank Accession Number: NM_031181.2
• Gene Symbol: Siglec-E
• Gene ID (NCBI): 83382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q91Y57
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily that contain sialoside-binding N-terminal domains (PMID: PMID: 9498912; 22749793). Mouse Siglec‑E, also known as myeloid inhibitory siglec (MIS), is a mouse CD33-related siglec that contains three extracellular immunoglobulin-like domains, a transmembrane region and a cytoplasmic tail bearing two immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which can recruit SHP-1 and SHP-2, two inhibitory regulators of immunoreceptor signal transduction (PMID: 11171044). Siglec-E is an inhibitory receptor and is expressed broadly on cells of innate immune cells including neutrophils, monocytes, macrophages, dendritic cells, and a subset of natural killer cells (PMID: 15048729; 31546700).