TBCD Recombinant monoclonal antibody

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  WB analysis using 82911-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82911-1-RR (TBCD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 82911-1-RR

  WB result of TBCD antibody (82911-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBCD transfected MCF-7 cells.

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  IF Staining of A549 using 82911-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TBCD antibody (82911-1-RR, Clone: 230209H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of MCF-7 using 82911-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TBCD Recombinant antibody (82911-1-RR, Clone:230209H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  Affinity and Kinetic Characterization of 82911-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82911-1 -RR against Human TBCD were performed. The affinity constant is 1.52 nM.

• Featured Product
• KD/KO Validated

TBCD Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 82911-1-RR

Clone No.230209H7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

230209H7, Beta-tubulin cofactor D, KIAA0988, PP1096, SSD1

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, HeLa cells, HepG2 cells
• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82911-1-RR targets TBCD in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6310

  Product name: Recombinant human TBCD protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-270 aa of BC003094

  Sequence: MALSDEPAAGGPEEEAEDETLAFGAALEAFGESAETRALLGRLREVHGGGAEREVALERFRVIMDKYQEQPHLLDPHLEWMMNLLLDIVQDQTSPASLVHLAFKFLYIITKVRGYKTFLRLFPHEVADVEPVLDLVTIQNPKDHEAWETRYMLLLWLSVTCLIPFDFSRLDGNLLTQPGQARMSIMDRILQIAESYLIVSDKARDAAAVLVSRFITRPDVKQSKMAEFLDWSLCNLARSSFQTMQGVITMDGTLQALAQIFKHGKREDCL

• Full Name: tubulin folding cofactor D
• Calculated Molecular Weight: 133 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC003094
• Gene Symbol: TBCD
• Gene ID (NCBI): 6904
• RRID: AB_3670642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BTW9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.