CoraLite® Plus 488-conjugated TEAD4 Recombinant antibody

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  IF Staining of HeLa using CL488-84932-4

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TEAD4 antibody (CL488-84932-4, Clone: 242496B2 ) at dilution of 1:200, CL594-Phalloidin (red).

TEAD4 Recombinant Antibody for IF/ICC

Cat No. CL488-84932-4

Clone No.242496B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

242496B2, EFTR 2, hRTEF 1B, RTEF1, TCF13L1

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84932-4 targets TEAD4 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TEAD4 fusion protein Ag3059
• Full Name: TEA domain family member 4
• Calculated Molecular Weight: 434 aa, 48 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC015497
• Gene Symbol: TEAD4
• Gene ID (NCBI): 7004
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15561
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TEAD4 belongs to a protein family that shares the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins, a TBP-associated factor, and a limiting transcriptional intermediary factor. TEAD4 also performs an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration, and epithelial-mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but the molecular weight of TEAD4 is detected at about 50-55 kDa.