TGF Beta 1 Polyclonal antibody

TGF Beta 1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18978-1-AP

Synonyms

CED, DPD1, LAP, TGF beta 1, TGFB, TGFB1, TGFbeta, TGF-beta1, TGFβ, TGFβ1, TGF-β1



Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18978-1-AP targets TGF Beta 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity buffloa, dog, human, miniping, mouse, pig, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name transforming growth factor, beta 1
Calculated molecular weight 44 kDa
Observed molecular weight 25 kDa
GenBank accession numberNM_000660
Gene symbol TGFB1
Gene ID (NCBI) 7040
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1). Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).

Protocols

Product Specific Protocols
WB protocol for TGF Beta 1 antibody 18978-1-APDownload protocol
IHC protocol for TGF Beta 1 antibody 18978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Br J Dermatol

Intense Foxp3(+) CD25(+) regulatory T cell infiltration is associated with high-grade cutaneous squamous cell carcinoma and counterbalanced by CD8(+) /Foxp3(+) CD25(+) ratio.

Authors - B Azzimonti
ratWB

Respir Physiol Neurobiol

Maternal high-fat diet feeding during pregnancy and lactation augments lung inflammation and remodeling in the offspring.

Authors - Ye Song
mouseWB

Pharmacology

Astaxanthin Attenuates Adriamycin-Induced Focal Segmental Glomerulosclerosis.

Authors - Guoyong Liu
humanIHC

Biomed Res Int

Epithelial-mesenchymal transition in keratocystic odontogenic tumor: possible role in locally aggressive behavior.

Authors - Wen-Qun Zhong
mouseWB

Life Sci

Slit2-Robo2 signaling modulates the fibrogenic activity and migration of hepatic stellate cells.

Authors - Zhiping Zeng
mouseWB

Diabetes Metab Syndr Obes

A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation.

Authors - CongXiao Zhang