|Positive WB detected in||MCF-7 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18978-1-AP targets TGF Beta 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||buffloa, canine, human, minipig, mouse, rat, sheep, swine|
|Host / Isotype||Rabbit / IgG|
|Full Name||transforming growth factor, beta 1|
|Calculated molecular weight||44 kDa|
|Observed molecular weight||25 kDa|
|GenBank accession number||NM_000660|
|Gene ID (NCBI)||7040|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1). Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).
Br J Dermatol
Intense Foxp3(+) CD25(+) regulatory T cell infiltration is associated with high-grade cutaneous squamous cell carcinoma and counterbalanced by CD8(+) /Foxp3(+) CD25(+) ratio.
Respir Physiol Neurobiol
Maternal high-fat diet feeding during pregnancy and lactation augments lung inflammation and remodeling in the offspring.
Astaxanthin Attenuates Adriamycin-Induced Focal Segmental Glomerulosclerosis.
Biomed Res Int
Epithelial-mesenchymal transition in keratocystic odontogenic tumor: possible role in locally aggressive behavior.
Slit2-Robo2 signaling modulates the fibrogenic activity and migration of hepatic stellate cells.
Diabetes Metab Syndr Obes
A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation.