Filter: AllWBIHC

WB analysis using 67831-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.
WB analysis of human placenta using 67831-1-Ig
human placenta tissue were subjected to SDS PAGE followed by western blot with 67831-1-Ig (THBD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 67831-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 67831-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 67831-1-Ig
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67831-1-Ig (CD141/Thrombomodulin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 67831-1-Ig
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67831-1-Ig (CD141/Thrombomodulin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 67831-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67831-1-Ig (CD141/Thrombomodulin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD141/Thrombomodulin Monoclonal antibody

CD141/Thrombomodulin Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67831-1-Ig

Clone No.5A4C5

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, human placenta tissue, THP-1 cells, HUVEC cells
Positive IHC detected in	human lung cancer tissue, human placenta tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67831-1-Ig targets CD141/Thrombomodulin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag5723 Product name: Recombinant human THBD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 18-350 aa of BC035602 Sequence: PAPAEPQPGGSQCVEHDCFALYPGPATFLNASQICDGLRGHLMTVRSSVAADVISLLLNGDGGVGRRRLWIGLQLPPGCGDPKRLGPLRGFQWVTGDNNTSYSRWARLDLNGAPLCGPLCVAVSAAEATVPSEPIWEEQQCEVKADGFLCEFHFPATCRPLAVEPGAAAAAVSITYGTPFAARGADFQALPVGSSAAVAPLGLQLMCTAPPGAVQGHWAREAPGAWDCSVENGGCEHACNAIPGAPRCQCPAGAALQADGRSCTASATQSCNDLCEHFCVPNPDQPGSYSCMCETGYRLAADQHRCEDVDDCILEPSPCPQRCVNTQGGFECH Predict reactive species
Full Name	thrombomodulin
Calculated Molecular Weight	60 kDa
Observed Molecular Weight	105 kDa
GenBank Accession Number	BC035602
Gene Symbol	THBD
Gene ID (NCBI)	7056
RRID	AB_2918593
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P07204
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thrombomodulin, also known as THBD and CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).