Anti-Human TIM3 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98115-1-RR

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit RecAb (98115-1-RR, Clone: 241422H1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD4 (OKT4) (APC-65134, Clone: OKT4). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98115-1-RR

  1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98115-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98115-1-RR against Human TIM3 were performed. The affinity constant is 0.688 nM.

TIM3 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98115-1-RR

Clone No.241422H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

241422H1, CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98115-1-RR targets TIM3 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: hepatitis A virus cellular receptor 2
• Calculated Molecular Weight: 301 aa, 33 kDa
• GenBank Accession Number: BC020843
• Gene Symbol: TIM3
• Gene ID (NCBI): 84868
• RRID: AB_3672262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8TDQ0
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920).