FITC Plus Anti-Human TIM3 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using FITC-98115

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD4 and 5 ul FITC Plus Anti-Human TIM3 Rabbit RecAb (FITC-98115, Clone: 241422H1). Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-98115

  1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human TIM3 Rabbit RecAb (FITC-98115, Clone: 241422H1) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human TIM3 Rabbit RecAb (FITC-98115, Clone: 241422H1) (black, dashed). Cells were not fixed.

TIM3 Uni-rAb^TM Recombinant Antibody for FC

Cat No. FITC-98115

Clone No.241422H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

241422H1, CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98115 targets TIM3 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: hepatitis A virus cellular receptor 2
• Calculated Molecular Weight: 301 aa, 33 kDa
• GenBank Accession Number: BC020843
• Gene Symbol: TIM3
• Gene ID (NCBI): 84868
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TDQ0
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920).