Tested Applications
| Positive WB detected in | HUVEC cells, HeLa cells, NIH/3T3 cells, C6 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells, HeLa cells, HepG2 cells, A549 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14168-1-AP targets Talin-1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5367 Product name: Recombinant human TLN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2193-2541 aa of BC042923 Sequence: GNSCRQEDVIATANLSRRAIADMLRACKEAAYHPEVAPDVRLRALHYGRECANGYLELLDHVLLTLQKPSPELKQQLTGHSKRVAGSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANALDDGQWSQGLISAARMVAAATNNLCEAANAAVQGHASQEKLISSAKQVAASTAQLLVACKVKADQDSEAMKRLQAAGNAVKRASDNLVKAAQKAAAFEEQENETVVVKEKMVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELRDEH Predict reactive species |
| Full Name | talin 1 |
| Calculated Molecular Weight | 2541 aa, 270 kDa |
| Observed Molecular Weight | 250-270 kDa |
| GenBank Accession Number | BC042923 |
| Gene Symbol | Talin-1 |
| Gene ID (NCBI) | 7094 |
| RRID | AB_2271861 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y490 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Talin-1 antibody 14168-1-AP | Download protocol |
| IF protocol for Talin-1 antibody 14168-1-AP | Download protocol |
| IHC protocol for Talin-1 antibody 14168-1-AP | Download protocol |
| IP protocol for Talin-1 antibody 14168-1-AP | Download protocol |
| WB protocol for Talin-1 antibody 14168-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Talin-1 Polyclonal antibody (Cat# 14168-1-AP) has 49 citations across journals including Nature Cell Biology, Nature Communications, ACS Nano, Advanced Science, PNAS, Cell Reports, eLife, and Biomaterials. Research fields span cancer biology (colorectal, esophageal, glioma, breast, pancreatic), cell adhesion and mechanotransduction, platelet function and thrombosis, renal fibrosis, wound healing, bone regeneration, cardiovascular disease, reproductive biology, viral infections, and disulfidptosis.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol The mitochondria enzyme OGDH defends against disulfidptosis by licensing METTL3-regulated NRF2 translation | ||
Nat Commun Intermetallics triggering pyroptosis and disulfidptosis in cancer cells promote anti-tumor immunity | ||
ACS Nano Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes. | ||
ACS Nano Robust and Regular Micronano Binary Texture on the Complex Curved Surface for Enhanced Reendothelialization and Antithrombotic Performance | ||
Adv Sci (Weinh) Granulosa Cell-Layer Stiffening Prevents Escape of Mural Granulosa Cells from the Post-Ovulatory Follicle |
Reviews
What customers say
All three reviews rate this antibody 5 stars. Users report excellent performance in Western blot, with clear and strong bands even at low dilutions (1:2000–1:5000). It also works well for immunofluorescence, with one user noting good staining at 1:200 overnight at 4 °C using Alexa Fluor 488. Tested across melanoma cells, MSCs, and human airway smooth muscle cells, the antibody consistently delivers reliable results in both WB and IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hunter (Verified Customer) (01-30-2026) | Fantastic antibody, very clear and strong bands at a low dilution.
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Udesh (Verified Customer) (08-12-2025) | Worked well for both WB and IF
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Elham (Verified Customer) (05-09-2025) | Really good staining. Used 1:200 dilution overnight at 4C and stained using Alexa Fluor 488 and actin Cell Mask 647
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