• Featured Product
  • KD/KO Validated

TLR2 Monoclonal antibody

TLR2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (3)

Applications

WB, Cell treatment

Conjugate

Unconjugated

CloneNo.

1C1D3

Cat no : 66645-1-Ig

Synonyms

CD282, TIL4, TLR2, toll like receptor 2



Tested Applications

Positive WB detected inTHP-1 cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

66645-1-Ig targets TLR2 in WB, Cell treatment applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TLR2 fusion protein Ag25626
Full Name toll-like receptor 2
Calculated molecular weight 784 aa, 90 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC033756
Gene symbol TLR2
Gene ID (NCBI) 7097
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLR2, also known as CD282, is a member of the Toll-like receptor (TLR) family. TLR receptors are type I integral transmembrane glycoproteins which play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs) and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).

Protocols

Product Specific Protocols
WB protocol for TLR2 antibody 66645-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

ACS Omega

Azilsartan Suppressed LPS-Induced Inflammation in U937 Macrophages through Suppressing Oxidative Stress and Inhibiting the TLR2/MyD88 Signal Pathway.

Authors - Qinglian Dong
mouseWB,Cell treatment

Front Pharmacol

Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway

Authors - Sairong Fan
pigWB

Cells

Clostridium Butyricum ZJU-F1 Benefits the Intestinal Barrier Function and Immune Response Associated with Its Modulation of Gut Microbiota in Weaned Piglets.

Authors - Jie Fu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

  • Applications: Other,