Tested Applications
| Positive WB detected in | THP-1 cells, U-937 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66645-1-Ig targets TLR2 in WB, IHC, IF/ICC, ELISA, Blocking assay, Western Blot applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25626 Product name: Recombinant human TLR2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 19-302 aa of BC033756 Sequence: KEESSNQASLSCDRNGICKGSSGSLNSIPSGLTEAVKSLDLSNNRITYISNSDLQRCVNLQALVLTSNGINTIEEDSFSSLGSLEHLDLSYNYLSNLSSSWFKPLSSLTFLNLLGNPYKTLGETSLFSHLTKLQILRVGNMDTFTKIQRKDFAGLTFLEELEIDASDLQSYEPKSLKSIQNVSHLILHMKQHILLLEIFVDVTSSVECLELRDTDLDTFHFSELSTGETNSLIKKFTFRNVKITDESLFQVMKLLNQISGLLELEFDDCTLNGVGNFRASDNDR Predict reactive species |
| Full Name | toll-like receptor 2 |
| Calculated Molecular Weight | 784 aa, 90 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC033756 |
| Gene Symbol | TLR2 |
| Gene ID (NCBI) | 7097 |
| RRID | AB_2882003 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O60603 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TLR2, also known as CD282, is a Toll-like receptor (TLR) family member. TLR receptors are type I integral transmembrane glycoproteins that play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs), and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens, and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TLR2 antibody 66645-1-Ig | Download protocol |
| IHC protocol for TLR2 antibody 66645-1-Ig | Download protocol |
| WB protocol for TLR2 antibody 66645-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
TLR2 antibody (Cat# 66645-1-Ig) has 50 citations across journals including Signal Transduction and Targeted Therapy, Advanced Science, Journal of Controlled Release, International Journal of Biological Sciences, Cell Reports, Brain Behavior and Immunity, Free Radical Biology and Medicine, Clinical and Translational Medicine, Journal of Investigative Dermatology, Cancer Cell International, Frontiers in Immunology, Journal of Ethnopharmacology, FASEB Journal, iScience, and others. Research fields span immunology/inflammation (TLR2–NF-κB/NLRP3 pathways), neurodegeneration, oncology, infectious disease, metabolic disorders, and cardiovascular/pulmonary/renal pathology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Innovative peptide-loaded hybrid membrane nanovaccine enables precise personalized immunotherapy for HPV-related cervical diseases. | ||
Adv Sci (Weinh) POSTN-Mediated Interplay of M1 Polarized Macrophage with Tendon-Derived Stem Cells to Drive Traumatic Heterotopic Ossification Formation through PTK7/ATK Signaling | ||
Int J Biol Sci NAT10 Promotes Tubular Epithelial Cell Senescence in Cisplatin-Induced Acute Kidney Injury by Regulating DDX17. | ||
Cell Commun Signal Calreticulin in the nasal mucus promotes neutrophil migration and pathogen clearance. | ||
NPJ Biofilms Microbiomes Naringin alleviates periodontitis via direct AMPK/Nrf2 activation and NLRP3 inhibition, amplified by gut microbiota/Arg-Gln modulation. |
Reviews
What customers say
One reviewer (Carly G.) rated this product 4/5 stars in a 2019 review. She used it in an antibody array to identify proteins in human EDTA plasma (lot 10006026). The overall feedback is positive, with the antibody performing well in a non-standard application beyond its primary intended use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Carly (Verified Customer) (09-27-2019) | Used in an antibody array to identify proteins in human EDTA plasma.
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