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TLR2 Monoclonal antibody

TLR2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


Human and More (2)







Cat no : 66645-1-Ig


CD282, TIL4, TLR2, toll like receptor 2

Tested Applications

Positive WB detected inTHP-1 cells, U-937 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inTHP-1 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66645-1-Ig targets TLR2 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TLR2 fusion protein Ag25626
Full Name toll-like receptor 2
Calculated Molecular Weight 784 aa, 90 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC033756
Gene Symbol TLR2
Gene ID (NCBI) 7097
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLR2, also known as CD282, is a member of the Toll-like receptor (TLR) family. TLR receptors are type I integral transmembrane glycoproteins which play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs) and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).


Product Specific Protocols
WB protocol for TLR2 antibody 66645-1-IgDownload protocol
IHC protocol for TLR2 antibody 66645-1-IgDownload protocol
IF protocol for TLR2 antibody 66645-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Clin Transl Med

Monocyte biomarkers define sargramostim treatment outcomes for Parkinson's disease.

Authors - Mai M Abdelmoaty

Life Sci

The novel mechanism of human norovirus induced diarrhea: Activation of PKD2 caused by HuNoVs destroyed AQP3 expression through AP2γ in intestinal epithelial cells

Authors - Ling Huang

Microbes Infect

Corydalis Saxicola Bunting Total Alkaloid Eliminates Porphyromonas gingivalis strain 33277 Internalized into Macrophages by Inhibition of TLR2

Authors - Lan Yang

Front Microbiol

PPARγ Ameliorates Mycobacterium tuberculosis H37Ra-Induced Foamy Macrophage Formation via the ABCG1-Dependent Cholesterol Efflux Pathway in THP-1 Macrophages.

Authors - Yutao Ye

ACS Omega

Azilsartan Suppressed LPS-Induced Inflammation in U937 Macrophages through Suppressing Oxidative Stress and Inhibiting the TLR2/MyD88 Signal Pathway.

Authors - Qinglian Dong


p53 suppresses the inflammatory response following respiratory syncytial virus infection by inhibiting TLR2

Authors - Jiao Liu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

  • Applications: Other,