TMEM165 Polyclonal antibody, PBS Only

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• TMEM165 Polyclonal antibody, PBS Only (20485-1-PBS)

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  WB analysis of Jurkat using 20485-1-AP (same clone as 20485-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

  IP result of anti-TMEM165 (IP:20485-1-AP, 4ug; Detection:20485-1-AP 1:1000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 20485-1-AP (same clone as 20485-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TMEM165 antibody (20485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM165 antibody (20485-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM165 antibody (20485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

TMEM165 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 20485-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Putative divalent cation/proton antiporter TMEM165, TMPT27, TPARL, Transmembrane protein 165, Transmembrane protein PT27

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Product Information

20485-1-PBS targets TMEM165 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14362

  Product name: Recombinant human TMEM165 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 61-177 aa of BC003545

  Sequence: VSTVLFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLLNGPGDVETGTSITVPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHC

• Full Name: transmembrane protein 165
• Calculated Molecular Weight: 324 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC003545
• Gene Symbol: TMEM165
• Gene ID (NCBI): 55858
• RRID: AB_10695184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HC07
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TMEM165 is a putative divalent cation:proton antiporter that exchanges calcium or manganese ions for protons across the Golgi membrane. It mediates the reversible transport of calcium or manganese to the Golgi lumen driven by the proton gradient and possibly the membrane potential generated by V-ATPase, and provides calcium or manganese cofactors to resident Golgi enzymes and contributes to the maintenance of an acidic luminal Golgi pH required for proper functioning of the secretory pathway (PMID:22683087, 23569283, 27008884, 32047108). TMEM165 has two isoforms, with molecular weights of 28 kDa and 35 kDa respectively. It is also subject to ubiquitination modification, which may cause an increase in its molecular weight.