TNIK Monoclonal antibody

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  WB analysis of K-562 using 67948-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood platelets using 67948-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 67948-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 67948-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 67948-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TNIK antibody (67948-1-Ig, Clone: 1E9H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of A431 using 67948-1-Ig

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TNIK (67948-1-Ig, Clone:1E9H3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

TNIK Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 67948-1-Ig

Clone No.1E9H3

Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

1E9H3, EC:2.7.11.1, KIAA0551, TRAF2 and NCK-interacting protein kinase

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Tested Applications

• Positive WB detected in: human peripheral blood platelets, K-562 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

67948-1-Ig targets TNIK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30710

  Product name: Recombinant human TNIK protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 157-538 aa of BC055427

  Sequence: PRVYQTSPTDEDEEDEESSATALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW

• Full Name: TRAF2 and NCK interacting kinase
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 155~180 kDa
• GenBank Accession Number: BC055427
• Gene Symbol: TNIK
• Gene ID (NCBI): 23043
• RRID: AB_2918700
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UKE5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Traf2- and Nck-interacting kinase (TNIK) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension (PMID: 24566388). TNIK is an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462).Western blot analysis detected TNIK at an apparent molecular mass of 150~180 kDa as cited in literature (PMID: 24566388).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Front Mol Biosci; Comprehensive analysis of senescence-related genes and immune infiltration in intervertebral disc degeneration: a meta-data approach utilizing bulk and single-cell RNA sequencing data; Authors - Ya-Jun Deng