|Positive WB detected in||mouse skeletal muscle tissue, human stomach tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human skeletal muscle tissue, human heart tissue, human lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||U2OS|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : |
|Sample-dependent, check data in validation data gallery|
13504-1-AP targets TNNC1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TNNC1 fusion protein Ag4339|
|Full Name||troponin C type 1 (slow)|
|Calculated molecular weight||161 aa, 18 kDa|
|Observed molecular weight||18 kDa|
|GenBank accession number||BC030244|
|Gene ID (NCBI)||7134|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. TnC plays an important role in excitation-contraction coupling and is the primary target of compounds such as levosimendan that augment Ca2+ responsiveness of the thin filament.
|Product Specific Protocols|
|WB protocol for TNNC1 antibody 13504-1-AP||Download protocol|
|IHC protocol for TNNC1 antibody 13504-1-AP||Download protocol|
|IF protocol for TNNC1 antibody 13504-1-AP||Download protocol|
|IP protocol for TNNC1 antibody 13504-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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