• Featured Product
  • KD/KO Validated

TNNC1 Polyclonal antibody

TNNC1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13504-1-AP

Synonyms

CMD1Z, TN C, TNC, TNNC, TNNC1, troponin C type 1 (slow)



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human stomach tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF :
Sample-dependent, check data in validation data gallery

Product Information

13504-1-AP targets TNNC1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNNC1 fusion protein Ag4339
Full Name troponin C type 1 (slow)
Calculated molecular weight 161 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC030244
Gene symbol TNNC1
Gene ID (NCBI) 7134
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. TnC plays an important role in excitation-contraction coupling and is the primary target of compounds such as levosimendan that augment Ca2+ responsiveness of the thin filament.

Protocols

Product Specific Protocols
WB protocol for TNNC1 antibody 13504-1-APDownload protocol
IHC protocol for TNNC1 antibody 13504-1-APDownload protocol
IF protocol for TNNC1 antibody 13504-1-APDownload protocol
IP protocol for TNNC1 antibody 13504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Med Sci Monit

TNNC1 Reduced Gemcitabine Sensitivity of Nonsmall-Cell Lung Cancer by Increasing Autophagy.

Authors - Xian Ye
humanWB

Onco Targets Ther

LukS-PV Inhibits Hepatocellular Carcinoma Cells Migration via the TNNC1/PI3K/AKT Axis.

Authors - Fan Ma
mouseWB

Mol Cell Biol

MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD.

Authors - Magdalena A Cichewicz
ratWB

J Proteome Res

iTRAQ-based proteomic analysis reveals recovery of impaired mitochondrial function in ischemic myocardium by Shenmai formula.

Authors - Yi Wang
humanWB

Nat Commun

Calcium-dependent FAK/CREB/TNNC1 signalling mediates the effect of stromal MFAP5 on ovarian cancer metastatic potential.

Authors - Cecilia S Leung
  • KD Validated