Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, Jurkat cells, HeLa cells, HEK-293 cells, K-562 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67829-1-Ig targets TOE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28982 Product name: Recombinant human TOE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-262 aa of BC009364 Sequence: MAADSDDGAVSAPAASDGGVSKSTTSGEELVVQVPVVDVQSNNFKEMWPSLLLAIKTANFVAVDTELSGLGDRKSLLNQCIEERYKAVCHAARTRSILSLGLACFKRQPDKGEHSYLAQVFNLTLLCMEEYVIEPKSVQFLIQHGFNFNQQYAQGIPYHKGNDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPESLGTFTADLCEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAG Predict reactive species |
| Full Name | target of EGR1, member 1 (nuclear) |
| Calculated Molecular Weight | 510 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC009364 |
| Gene Symbol | TOE1 |
| Gene ID (NCBI) | 114034 |
| RRID | AB_2918591 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96GM8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TOE1 antibody 67829-1-Ig | Download protocol |
| IHC protocol for TOE1 antibody 67829-1-Ig | Download protocol |
| WB protocol for TOE1 antibody 67829-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TOE1 Monoclonal antibody (2C10H7), catalog number 67829-1-Ig, has 1 total citation. The citation appears in Stem Cell Reports (Impact Factor 5.6), published in 2026. The study investigated TOE1 as a β-catenin interacting protein regulating hematopoietic cell proliferation through PAK2 modulation, placing it in the fields of stem cell biology and hematopoiesis. The antibody was used for immunofluorescence (IF) in human samples.
| Species | Application | Title |
|---|---|---|
Stem Cell Reports TOE1 is a β-catenin interacting protein regulating the proliferation of hematopoietic cells through PAK2 modulation.
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