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CoraLite® Plus 488-conjugated TOE1 Monoclonal antibody

TOE1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C10H7

Cat no : CL488-67829

Synonyms

FLJ13949, Target of EGR1 protein 1, TOE1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated TOE1 Monoclonal anti (CL488-67829)

    IF Staining of HepG2 using CL488-67829

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TOE1 antibody (CL488-67829, Clone: 2C10H7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated TOE1 Monoclonal anti (CL488-67829)

    FC experiment of HepG2 using CL488-67829

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TOE1 (CL488-67829, Clone:2C10H7) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67829 targets TOE1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TOE1 fusion protein Ag28982
Full Name target of EGR1, member 1 (nuclear)
Calculated Molecular Weight 510 aa, 57 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC009364
Gene Symbol TOE1
Gene ID (NCBI) 114034
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TOE1 antibody CL488-67829Download protocol
FC protocol for CL Plus 488 TOE1 antibody CL488-67829Download protocol
Standard Protocols
Click here to view our Standard Protocols