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TOM1L2 Polyclonal antibody, PBS Only (32179-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 32179-1-AP (same clone as 32179-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 32179-1-AP (same clone as 32179-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.
WB analysis using 32179-1-AP (same clone as 32179-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 32179-1-AP (same clone as 32179-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32179-1-AP (TOM1L2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 32179-1-AP (same clone as 32179-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32179-1-AP (TOM1L2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 32179-1-AP (same clone as 32179-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TOM1L2 antibody (32179-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

TOM1L2 Polyclonal antibody, PBS Only

Name: TOM1L2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 32179-1-PBS

TOM1L2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 32179-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Target Of Myb1 Like 2 Membrane Trafficking Protein, Target of Myb-like protein 2, TOM1-like protein 2

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

32179-1-PBS targets TOM1L2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag37612 Product name: Recombinant human TOM1L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 300-457 aa of NM_001082968.1 Sequence: LRYERFERYRSGRSVQNASNGVLNEVTEDNLIDLGPGSPAVVSPMVGNTAPPSSLSSQLAGLDLGTESVSGTLSSLQQCNPRDGFDMFAQTRGNSLAEQRKTVTYEDPQAVGGLASALDNRKQSSEGIPVAQPSVMDDIEVWLRTDLKGDDLEEGVTS Predict reactive species
Full Name	target of myb1-like 2 (chicken)
Calculated Molecular Weight	56 kDa，507aa
Observed Molecular Weight	60 kDa
GenBank Accession Number	NM_001082968.1
Gene Symbol	TOM1L2
Gene ID (NCBI)	146691
RRID	AB_3670215
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	Q6ZVM7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Target of Myb-like protein 2 (TOM1L2) acts as a MYO6/Myosin VI adapter protein that targets myosin VI to endocytic structures (PMID: 23023224). It may play a role in recruiting clathrin to endosomes and regulating growth factor-induced mitogenic signaling (PMID: 16412388; 16479011). TOM1L2 also regulates membrane trafficking that is linked to immunity and cell proliferation (PMID: 20604899).