|Positive WB detected in||HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66777-1-Ig targets TOM20 in WB, IHC, IF, FC,ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||TOM20 fusion protein Ag2378|
|Full Name||translocase of outer mitochondrial membrane 20 homolog (yeast)|
|Calculated molecular weight||145 aa, 16 kDa|
|Observed molecular weight||16 kDa|
|GenBank accession number||BC000882|
|Gene ID (NCBI)||9804|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.
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