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TOM70 Polyclonal antibody

TOM70 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 14528-1-AP



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human kidney tissue, mouse heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

14528-1-AP targets TOM70 in WB, IP, IHC, IF, FC, CoIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOM70 fusion protein Ag6027
Full Name translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC052994
Gene symbol TOMM70A
Gene ID (NCBI) 9868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOMM70A (or TOM70) is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.


Product Specific Protocols
WB protocol for TOM70 antibody 14528-1-APDownload protocol
IHC protocol for TOM70 antibody 14528-1-APDownload protocol
IF protocol for TOM70 antibody 14528-1-APDownload protocol
IP protocol for TOM70 antibody 14528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



JCI Insight

Mitophagy and mitochondrial biogenesis in atrial tissue of patients undergoing heart surgery with cardiopulmonary bypass.

Authors - Allen M Andres

Am J Physiol Cell Physiol

Yap regulates mitochondrial structure remodeling during myoblast differentiation.

Authors - Shiyuan Huang

Cell Rep

A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.

Authors - Hezhi Fang

Int J Mol Sci

The Mechanism of Mitochondrial Injury in Alpha-1 Antitrypsin Deficiency Mediated Liver Disease.

Authors - Nazli Khodayari


Hypothermia promotes mitochondrial elongation In cardiac cells via inhibition of Drp1.

Authors - David Taylor

PLoS One

Differential responses to folic acid in an established keloid fibroblast cell line are mediated by JAK1/2 and STAT3.

Authors - Katelyn J McCann


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tom (Verified Customer) (04-15-2021)

Cells firxed with 3.7% paraformaldehyde. Antibody visualized using anti-rabbit Alexa Fluor 647 secondary.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cos7
TOM70 Antibody Immunofluorescence validation (1:100 dilution) in Cos7 (Cat no:14528-1-AP)

Daniela (Verified Customer) (09-10-2019)

Used for Western Blot on total cell lysate from HeLa, this antibody identifies a strong band slightly below 75 kDa (proteins run on 15% acrylamide gel) and several fainter no-specific bands.Used for co-immunoprecipitation to detect over-expressed Flag-tagged protein interacting with Tom70, it identifies a sharp single band at the expected MW.

  • Applications: Western Blot, Immunoprecipitation, Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells