TOP2A Monoclonal antibody

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  WB analysis using 66541-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66541-1-Ig (TOP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66541-1-Ig

  WB result of TOP2A antibody (66541-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A transfected HepG2 cells.

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  IHC staining of human lung cancer using 66541-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66541-1-Ig (TOP2A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66541-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66541-1-Ig (TOP2A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66541-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66541-1-Ig (TOP2A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

TOP2A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66541-1-Ig

Clone No.1E12B3

Publications(4)

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DNA topoisomerase 2 alpha, TOP2, TOP2A, TP2A

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, L02 cells, Jurkat cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2500-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 3 publications below

Product Information

66541-1-Ig targets TOP2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19551

  Product name: Recombinant human TOP2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1187-1531 aa of BC140791

  Sequence: QDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF

• Full Name: topoisomerase (DNA) II alpha 170kDa
• Calculated Molecular Weight: 1531 aa, 174 kDa
• Observed Molecular Weight: 174 kDa
• GenBank Accession Number: BC140791
• Gene Symbol: TOP2A
• Gene ID (NCBI): 7153
• RRID: AB_2881903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11388
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Aging (Albany NY); Identification of differentially expressed genes in non-small cell lung cancer.; Authors - Ke Wang
human	WB,IHC	Front Oncol; TOP2A correlates with poor prognosis and affects radioresistance of medulloblastoma; Authors - Yufeng Zhang ; KD Validated
human	WB,IHC	Reprod Biol Endocrinol; TOP2A deficit-induced abnormal decidualization leads to recurrent implantation failure via the NF-κB signaling pathway; Authors - Huijia Fu ; KD Validated
human	WB	Front Mol Biosci; HJURP modulates cell proliferation and chemoresistance via the MYC/TOP2A transcriptional axis in gastric cancer; Authors - Xu Li