TOP2A Monoclonal antibody, PBS Only

• 

  WB analysis using 66541-1-Ig (same clone as 66541-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66541-1-Ig (TOP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66541-1-Ig (same clone as 66541-1-PBS)

  WB result of TOP2A antibody (66541-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A transfected HepG2 cells. This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66541-1-Ig (same clone as 66541-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66541-1-Ig (TOP2A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66541-1-Ig (same clone as 66541-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66541-1-Ig (TOP2A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66541-1-Ig (same clone as 66541-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66541-1-Ig (TOP2A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TOP2A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66541-1-PBS

Clone No.1E12B3

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DNA topoisomerase 2 alpha, TOP2, TOP2A, TP2A

---

Product Information

66541-1-PBS targets TOP2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19551

  Product name: Recombinant human TOP2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1187-1531 aa of BC140791

  Sequence: QDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF

• Full Name: topoisomerase (DNA) II alpha 170kDa
• Calculated Molecular Weight: 1531 aa, 174 kDa
• Observed Molecular Weight: 174 kDa
• GenBank Accession Number: BC140791
• Gene Symbol: TOP2A
• Gene ID (NCBI): 7153
• RRID: AB_2881903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11388
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.