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TOP2B Polyclonal antibody, PBS Only (20549-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 20549-1-AP (same clone as 20549-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)
WB result of TOP2B antibody (20549-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 20549-1-AP (same clone as 20549-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 20549-1-AP (same clone as 20549-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 20549-1-AP (same clone as 20549-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 20549-1-AP (same clone as 20549-1-PBS)
WB result of TOP2B antibody (20549-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HeLa cells. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)
WB result of TOP2B antibody (20549-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)
IP result of anti-TOP2B (IP:20549-1-AP, 4ug; Detection:20549-1-AP 1:300) with HepG2 cells lysate 3200ug. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 20549-1-AP (same clone as 20549-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 20549-1-AP (same clone as 20549-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 20549-1-AP (same clone as 20549-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 20549-1-AP (same clone as 20549-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 20549-1-AP (same clone as 20549-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody (20549-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)
Immunofluorescent analysis of HepG2 cells, using TOP2B antibody 20549-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

TOP2B Polyclonal antibody, PBS Only

Name: TOP2B Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 20549-1-PBS

TOP2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 20549-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

20549-1-PBS targets TOP2B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	topoisomerase (DNA) II beta 180kDa
Calculated Molecular Weight	183 kDa
Observed Molecular Weight	183 kDa
GenBank Accession Number	NM_001068
Gene Symbol	TOP2B
Gene ID (NCBI)	7155
RRID	AB_10700004
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q02880
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to TOP2B.