TPBG Polyclonal antibody, PBS Only

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• TPBG Polyclonal antibody, PBS Only (29394-1-PBS)

• 

  WB analysis using 29394-1-AP (same clone as 29394-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29394-1-AP (TPBG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29394-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 29394-1-AP (same clone as 29394-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 29394-1-AP (TPBG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29394-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 29394-1-AP (same clone as 29394-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 29394-1-AP (TPBG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29394-1-PBS in a different storage buffer formulation.

TPBG Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 29394-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

29394-1-PBS targets TPBG in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30019

  Product name: Recombinant human TPBG protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 153-355 aa of NM_006670

  Sequence: PLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPPSLQTS

• Full Name: trophoblast glycoprotein
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 72-80 kDa
• GenBank Accession Number: NM_006670
• Gene Symbol: TPBG
• Gene ID (NCBI): 7162
• RRID: AB_2918290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13641
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Trophoblast glycoprotein (TPBG), also termed 5T4, is a 72 kDa transmembrane glycoprotein and an extensively investigated oncofetal antigen, which is limited in normal adult tissues, but highly expressed in various types of cancer in human. TPBG is a novel PKCa-dependent phosphoprotein in retinal rod bipolar cells (RBCs). (PMID: 18097562, PMID: 31255707)