TPX2 Recombinant antibody

• 

  WB analysis using 85118-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85118-6-RR (TPX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 85118-6-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85118-6-RR (TPX2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 85118-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPX2 antibody (85118-6-RR, Clone: 242528G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 85118-6-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TPX2 Recombinant antibody (85118-6-RR, Clone:242528G6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 85118-6-RR

  Biolayer interferometry (BLl) kinetic assays of 85118-6-RR against Human TPX2 were performed. The affinity constant is 2.24 nM.

TPX2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 85118-6-RR

Clone No.242528G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

242528G6, C20orf1, C20orf2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL 2

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, SMMC-7721 cells, PANC-1 cells, NIH/3T3 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85118-6-RR targets TPX2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TPX2 fusion protein Ag2334
• Full Name: TPX2, microtubule-associated, homolog (Xenopus laevis)
• Calculated Molecular Weight: 747 aa, 86 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC020207
• Gene Symbol: TPX2
• Gene ID (NCBI): 22974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9ULW0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).