CoraLite® Plus 488-conjugated TPX2 Recombinant antibody

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  IF Staining of HepG2 using CL488-85118-6

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TPX2 antibody (CL488-85118-6, Clone: 242528G6 ) at dilution of 1:200, CL594-Phalloidin (red).

TPX2 Recombinant Antibody for IF/ICC

Cat No. CL488-85118-6

Clone No.242528G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

242528G6, C20orf1, C20orf2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL 2

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85118-6 targets TPX2 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TPX2 fusion protein Ag2334
• Full Name: TPX2, microtubule-associated, homolog (Xenopus laevis)
• Calculated Molecular Weight: 747 aa, 86 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC020207
• Gene Symbol: TPX2
• Gene ID (NCBI): 22974
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9ULW0
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).