TRAF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRAF2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 26846-1-AP

Synonyms

TRAF2, TRAP, TRAP3

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Validation Data Gallery

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  WB analysis of HepG2 using 26846-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26846-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 26846-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 26846-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 26846-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 26846-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26846-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 26846-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TRAF2 antibody (26846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 26846-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26846-1-AP (TRAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HUVEC using 26846-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 26846-1-AP (TRAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, human testis tissue, HeLa cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells, HeLa cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

26846-1-AP targets TRAF2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAF2 fusion protein Ag25387
• Full Name: TNF receptor-associated factor 2
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC032410
• Gene Symbol: TRAF2
• Gene ID (NCBI): 7186
• RRID: AB_2880656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
human	IHC	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu
mouse	WB	J Hazard Mater; Endoplasmic reticulum stress manipulates autophagic response that antagonizes polybrominated diphenyl ethers quinone induced cytotoxicity in microglial BV2 cells.; Authors - Qiushuang Zhu
human	WB	EMBO Rep; Snail enhances arginine synthesis by inhibiting ubiquitination-mediated degradation of ASS1.; Authors - Hao Jia ; KD Validated
human,mouse	WB,IHC,IF	Free Radic Biol Med; TRAF2 inhibits senescence in hepatocellular carcinoma cells via regulating the ROMO1/ NAD+/SIRT3/SOD2 axis; Authors - Jiping Yao ; KD Validated
mouse	IF	J Nanobiotechnology; The ketone body β-hydroxybutyrate alleviates CoCrMo alloy particles induced osteolysis by regulating NLRP3 inflammasome and osteoclast differentiation.; Authors - Yanglin Wu