CoraLite®594-conjugated TRIM21 Monoclonal antibody

TRIM21 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E8B9

Cat no : CL594-67136

Synonyms

52 kDa Ro protein, RING finger protein 81, RNF81, Ro(SS A), RO52, SS A, SSA, SSA1, TRIM21, tripartite motif containing 21

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-67136

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 TRIM21 antibody (CL594-67136, Clone: 1E8B9 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67136 targets TRIM21 in IF applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIM21 fusion protein Ag28377
• Full Name: tripartite motif-containing 21
• Calculated Molecular Weight: 475 aa, 54 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC010861
• Gene Symbol: TRIM21
• Gene ID (NCBI): 6737
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.