TRIM33 Polyclonal antibody, PBS Only

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• TRIM33 Polyclonal antibody, PBS Only (55374-1-PBS)

• 

  WB analysis of COLO 320 using 55374-1-AP (same clone as 55374-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 55374-1-AP (same clone as 55374-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 55374-1-AP (same clone as 55374-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 55374-1-AP (same clone as 55374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55374-1-AP (TRIM33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 55374-1-AP (same clone as 55374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55374-1-AP (TRIM33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TRIM33 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 55374-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

55374-1-PBS targets TRIM33 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tripartite motif-containing 33
• Calculated Molecular Weight: 122 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: NM_015906
• Gene Symbol: TRIM33
• Gene ID (NCBI): 51592
• RRID: AB_11183035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UPN9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRIM33, also named as KIAA1113, RFG7 and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight of detected is about 140-150 kDa as the same as the result from the paper(PMID:25639486;26592194).