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TRPM8 Polyclonal antibody, PBS Only (12813-1-PBS)

Filter: AllIHCIF/ICC

IHC staining of human liver cancer using 12813-1-AP (same clone as 12813-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12813-1-AP (same clone as 12813-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12813-1-AP (same clone as 12813-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12813-1-AP (same clone as 12813-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 12813-1-AP (same clone as 12813-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 12813-1-AP (same clone as 12813-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 12813-1-AP (same clone as 12813-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 12813-1-AP (same clone as 12813-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.
IF Staining of SH-SY5Y using 12813-1-AP (same clone as 12813-1-PBS)
Immunofluorescent analysis of SH-SY5Y cells using 12813-1-AP (TRPM8 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 12813-1-PBS in a different storage buffer formulation.

TRPM8 Polyclonal antibody, PBS Only

Name: TRPM8 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12813-1-PBS

TRPM8 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 12813-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

LTrpC 6, LTrpC6, LTrpC-6, Trp p8, TRPP8

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12813-1-PBS targets TRPM8 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3483 Product name: Recombinant human TRPM8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-192 aa of BC001135 Sequence: SFLPVHTIVLIRENVCKCGYAQSQHMEGTQINQSEKWNYKKHTKEFPTDAFGDIQFETLGKKGKYIRLSCDTDAEILYELLTQHWHLKTPNLVISVTGGAKNFALKPRMRKIFSRLIYIAQSKGAWILTGGTHYGLMKYIGEVVRDNTISRSSEENIVAIGIAAWGMVSNRDTLIRNCDAEVPVGQEEVC Predict reactive species
Full Name	transient receptor potential cation channel, subfamily M, member 8
Calculated Molecular Weight	1104 aa, 128 kDa
GenBank Accession Number	BC001135
Gene Symbol	TRPM8
Gene ID (NCBI)	79054
RRID	AB_2877883
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7Z2W7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TRPM8, also known as CMR1 (cold-menthol receptor-1), is a nonselective cation channel activated by cold and the cooling substances menthol and icilin (PMID: 11893340; 11882888). It serves as a neuronal sensor of cold temperatures. Functioning TRPM8 channels are required for behavioral responses to innocuous cool, noxious cold, injury-evoked cold hypersensitivity, and cooling-mediated analgesia (PMID: 17538622; 21438154; 16920620; 21984952). Additionally, TRPM8 has also been reported to be involved in thermoregulation and the regulation of basal tear flow (PMID: 21407809; 21076394). TRPM8 is expressed extensively in sensory nerves, human prostate and overexpressed in a variety of cancers including prostate, breast, lung, colon and skin melanomas (PMID: 11325849; 20816009).