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TSC22D2 Polyclonal antibody, PBS Only (25418-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 25418-1-AP (same clone as 25418-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.
WB analysis of MDA-MB-453s using 25418-1-AP (same clone as 25418-1-PBS)
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 25418-1-AP (same clone as 25418-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 25418-1-AP (same clone as 25418-1-PBS)
IP result of anti-TSC22D2 (IP:25418-1-AP, 5ug; Detection:25418-1-AP 1:300) with HepG2 cells lysate 2500ug. This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 25418-1-AP (same clone as 25418-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 25418-1-AP (same clone as 25418-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 25418-1-AP (same clone as 25418-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 25418-1-AP (same clone as 25418-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 25418-1-AP (same clone as 25418-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TSC22D2 antibody (25418-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 25418-1-PBS in a different storage buffer formulation.

TSC22D2 Polyclonal antibody, PBS Only

Name: TSC22D2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 25418-1-PBS

TSC22D2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 25418-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

TSC22 domain family protein 2, TILZ4c, TILZ4b, TILZ4a, TILZ4

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

25418-1-PBS targets TSC22D2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22294 Product name: Recombinant human TSC22D2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 190-543 aa of BC044643 Sequence: SDSSVLTRSGDCIRHSSTFDQTAERDSGLGATGGSVVVVVASMQGAHGPESGTDSSLTAVSQLPPSEKMSQPTPAQPQSFSVGQPQPPPPPVGGAVAQSSAPLPPFPGAATGPQPMMAAAQPSQPQGAGPGGQTLPPTNVTLAQPAMSLPPQPGPAVGAPAAQQPQQFAYPQPQIPPGHLLPVQPSGQSEYLQQHVAGLQPPSPAQPSSTGAAASPATAATLPVGTGQNASSVGAQLMGASSQPSEAMAPRTGPAQGGQVAPCQPTGVPPATVGGVVQPCLGPAGAGQPQSVPPPQMGGSGPLSAVPGGPHAVVPGVPNVPAAVPAPSVPSVSTTSVTMPNVPAPLAQSQQLSS Predict reactive species
Full Name	TSC22 domain family, member 2
Calculated Molecular Weight	780 aa, 79 kDa
Observed Molecular Weight	100-110 kDa
GenBank Accession Number	BC044643
Gene Symbol	TSC22D2
Gene ID (NCBI)	9819
RRID	AB_2880069
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75157
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TSC22D2 (transforming growth factor β-stimulated clone 22 domain family, member 2) is a member of the TSC-22 domain family comprising putative transcription factors that are characterized by a carboxy-terminal leucine zipper and an adjacent TSC-box. TSC22D2 is expressed at low levels in CRC and that TSC22D2 overexpression significantly inhibited the growth rate of cancer cells (PMID: 27573352).