TSG-6 Recombinant monoclonal antibody

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  WB analysis using 83163-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83163-4-RR (TNFAIP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 83163-4-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug TSG-6 Recombinant antibody (83163-4-RR, Clone:230438D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  Affinity and Kinetic Characterization of 83163-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83163-4-RR against Human TSG-6 were performed. The affinity constant is 1.93 nM.

TSG-6 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83163-4-RR

Clone No.230438D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

TNFAIP6, 230438D5, EC:3.1.1.-, Hyaluronate binding protein, Hyaluronate-binding protein

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, NIH/3T3 cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83163-4-RR targets TSG-6 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3885

  Product name: Recombinant human TNFAIP6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-277 aa of BC030205

  Sequence: MIILIYLFLLLWEDTQGWGFKDGIFHNSIWLERAAGVYHREARSGKYKLTYAEAKAVCEFEGGHLATYKQLEAARKIGFHVCAAGWMAKGRVGYPIVKPGPNCGFGKTGIIDYGIRLNRSERWDAYCYNPHAKECGGVFTDPKQIFKSPGFPNEYEDNQICYWHIRLKYGQRIHLSFLDFDLEDDPGCLADYVEIYDSYDDVHGFVGRYCGDELPDDIISTGNVMTLKFLSDASVTAGGFQIKYVAMDPVSKSSQGKNTSTTSTGNKNFLAGRFSHL

• Full Name: tumor necrosis factor, alpha-induced protein 6
• Calculated Molecular Weight: 277 aa, 31 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: BC030205
• Gene Symbol: TNFAIP6
• Gene ID (NCBI): 7130
• RRID: AB_3670858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P98066
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.