TXNIP Polyclonal antibody, PBS Only

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• TXNIP Polyclonal antibody, PBS Only (18243-1-PBS)

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  WB analysis of K-562 using 18243-1-AP (same clone as 18243-1-PBS)

  WB result of TXNIP antibody (18243-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNIP transfected K-562 cells. This data was developed using the same antibody clone with 18243-1-PBS in a different storage buffer formulation.

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  WB analysis using 18243-1-AP (same clone as 18243-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18243-1-AP (TXNIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18243-1-PBS in a different storage buffer formulation.

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  IP experiment of BxPC-3 using 18243-1-AP (same clone as 18243-1-PBS)

  IP result of anti-TXNIP (IP:18243-1-AP, 4ug; Detection:18243-1-AP 1:1000) with BxPC-3 cells lysate 1320 ug. This data was developed using the same antibody clone with 18243-1-PBS in a different storage buffer formulation.

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  IP experiment of HL-60 using 18243-1-AP (same clone as 18243-1-PBS)

  IP result of anti-TXNIP (IP:18243-1-AP, 4ug; Detection:18243-1-AP 1:1000) with HL-60 cells lysate 1480 ug. This data was developed using the same antibody clone with 18243-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 18243-1-AP (same clone as 18243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18243-1-AP (TXNIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18243-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 18243-1-AP (same clone as 18243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18243-1-AP (TXNIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18243-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18243-1-AP (same clone as 18243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18243-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18243-1-AP (same clone as 18243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18243-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 18243-1-AP (same clone as 18243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18243-1-AP (TXNIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18243-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 18243-1-AP (same clone as 18243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18243-1-AP (TXNIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18243-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 18243-1-AP (same clone as 18243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18243-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 18243-1-AP (same clone as 18243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18243-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TXNIP Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 18243-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Thioredoxin binding protein 2, thioredoxin interacting protein, Thioredoxin-binding protein 2, Thioredoxin-interacting protein, VDUP1

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Product Information

18243-1-PBS targets TXNIP in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13009

  Product name: Recombinant human TXNIP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-391 aa of BC093702

  Sequence: MVMFKKIKSFEVVFNDPEKVYGSGEKVAGRVIVEVCEVTRVKAVRILACGVAKVLWMQGSQQCKQTSEYLRYEDTLLLEDQPTGENEMVIMRPGNKYEYKFGFELPQGPLGTSFKGKYGCVDYWVKAFLDRPSQPTQETKKNFEVVDLVDVNTPDLMAPVSAKKEKKVSCMFIPDGRVSVSARIDRKGFCEGDEISIHADFENTCSRIVVPKAAIVARHTYLANGQTKVLTQKLSSVRGNHIISGTCASWRGKSLRVQKIRPSILGCNILRVEYSLLIYVSVPGSKKVILDLPLVIGSRSGLSSRTSSMASRTSSEMSWVDLNIPDTPEAPPCYMDVIPEDHRLESPTTPLLDDMDGSQDSPIFMYAPEFKFMPPPTYTEVDPCILNNNVQ

• Full Name: thioredoxin interacting protein
• Calculated Molecular Weight: 391 aa, 44 kDa
• Observed Molecular Weight: 44-55 kDa
• GenBank Accession Number: BC093702
• Gene Symbol: TXNIP
• Gene ID (NCBI): 10628
• RRID: AB_2876873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H3M7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TXNIP, also known as VDUP-1 or TBP-2, belongs to alpha-arrestin protein family and is perhaps the only family member known to bind thioredoxin (TRX). TXNIP was induced by Vitamin D3, but not induced by another monocyte or macrophage differentiation inducer: phorbol 12-myristate 13-acetate (PMA). TXNIP bound catalytic active center of thioredoxin (TRX), which protected cells against oxidative stress. TXNIP was found to be a negative regulator of thioredoxin activity and inducer of the intracellular level of reactive oxygen species (ROS). TXNIP plays an important role in a wide variety of biological functions, such as the regulation of cell death, growth, differentiation, and energy metabolism.