Testin Recombinant monoclonal antibody

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• Testin Recombinant monoclonal antibody (86013-3-RR)

• 

  WB analysis using 86013-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86013-3-RR (Testin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 86013-3-RR

  IP result of anti-Testin (IP:86013-3-RR, 4ug; Detection:86013-3-RR 1:1000) with K-562 cells lysate 2440 ug.

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  IF Staining of K-562 using 86013-3-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Testin antibody (86013-3-RR, Clone: 250432C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity of 86013-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86013-3-RR against Human Testin were performed. The affinity constant is 2.17 nM.

Testin Uni-rAb^® Recombinant Antibody for WB, IF/ICC, IP, ELISA

Cat No. 86013-3-RR

Clone No.250432C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

TES

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, K-562 cells, mouse testis tissue, rat testis tissue, human testis tissue
• Positive IP detected in: K-562 cells
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86013-3-RR targets Testin in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0379

  Product name: Recombinant human TES protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 108-323 aa of BC001451

  Sequence: TVTYEWAPPVQNQALARQYMQMLPKEKQPVAGSEGAQYRKKQLAKQLPAHDQDPSKCHELSPREVKEMEQFVKKYKSEALGVGDVKLPCEMDAQGPKQMNIPGGDRSTPAAVGAMEDKSAEHKRTQYSCYCCKLSMKEGDPAIYAERAGYDKLWHPACFVCSTCHELLVDMIYFWKNEKLYCGRHYCDSEKPRCAGCDELIFSNEYTQAENQNWHL

• Full Name: testis derived transcript (3 LIM domains)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 42-48 kDa
• GenBank Accession Number: BC001451
• Gene Symbol: TES
• Gene ID (NCBI): 26136
• RRID: AB_3744550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UGI8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Testin (TES) is a cytoskeleton-associated protein that localizes along actin stress fibers at cell-cell contact areas and at focal adhesion plaques. It interacts with a variety of cytoskeletal proteins and gets involved in regulation of cell adhesion and migration. TES is a putative tumor suppressor and reduced expression of TES has been reported in various cancers. In addition, TES is a marker for Sertoli-Germ Cell junction and is useful to monitor the disruption of intercellular junctions in the testis.