Tim23 Monoclonal antibody

Tim23 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

3H10F5

Cat no : 67535-1-Ig

Synonyms

PRO1197, TIM23, TIMM23

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Validation Data Gallery

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  WB analysis of A549 using 67535-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67535-1-Ig (Tim23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67535-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67535-1-Ig (Tim23 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67535-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67535-1-Ig (Tim23 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67535-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (Tim23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67535-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (Tim23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67535-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tim23 antibody (67535-1-Ig, Clone: 3H10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, NIH/3T3 cells, RAW 264.7 cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells, HSC-T6 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

67535-1-Ig targets Tim23 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Tim23 fusion protein Ag27069
• Full Name: translocase of inner mitochondrial membrane 23 homolog (yeast)
• Calculated molecular weight: 22 kDa
• Observed molecular weight: 21 kDa
• GenBank accession number: BC062707
• Gene symbol: TIMM23
• Gene ID (NCBI): 10431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.

Publications

Species	Application	Title
human	WB	Autophagy; The nucleoprotein of influenza A virus inhibits the innate immune response by inducing mitophagy; Authors - Bo Zhang
mouse	WB, IF	Autophagy; Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival.; Authors - Yinjuan Song