TIM23 Monoclonal antibody

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  WB analysis using 67535-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67535-1-Ig (Tim23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67535-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (Tim23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67535-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (Tim23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67535-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIM23 antibody (67535-1-Ig, Clone: 3H10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

TIM23 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67535-1-Ig

Clone No.3H10F5

Publications(7)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

67535, TIMM23, 3H10F5, Mitochondrial import inner membrane translocase subunit Tim23, TIMM 23

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Tested Applications

• Positive WB detected in: A549 cells, RAW 264.7 cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 1 publications below

Product Information

67535-1-Ig targets TIM23 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TIM23 fusion protein Ag27069
• Full Name: translocase of inner mitochondrial membrane 23
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC062707
• Gene Symbol: TIMM23
• Gene ID (NCBI): 100287932
• RRID: AB_2882754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O14925
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.

Publications

Species	Application	Title
human	WB	Autophagy; The nucleoprotein of influenza A virus inhibits the innate immune response by inducing mitophagy; Authors - Bo Zhang
mouse	WB,IF	Autophagy; Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival.; Authors - Yinjuan Song
mouse	WB	Mol Med; 4-Octyl itaconate attenuates LPS-induced acute kidney injury by activating Nrf2 and inhibiting STAT3 signaling; Authors - Lujun Xu
human	WB	Zool Res; Choline dehydrogenase interacts with SQSTM1 to activate mitophagy and promote coelomocyte survival in Apostichopus japonicus following Vibrio splendidus infection; Authors - Lian-Lian Sun
mouse,human	WB	Front Pharmacol; Low-dose of caffeine alleviates high altitude pulmonary edema via regulating mitochondrial quality control process in AT1 cells; Authors - Liuyang Tian
human	WB	Commun Biol; RABIF promotes hepatocellular carcinoma progression through regulation of mitophagy and glycolysis; Authors - Ning Feng