Tim23 Monoclonal antibody

Tim23 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3H10F5

Cat no : 67535-1-Ig

Synonyms

PRO1197, TIM23, TIMM23



Tested Applications

Positive WB detected inA549 cells, RAW 264.7 cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67535-1-Ig targets Tim23 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Tim23 fusion protein Ag27069
Full Name translocase of inner mitochondrial membrane 23 homolog (yeast)
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC062707
Gene Symbol TIMM23
Gene ID (NCBI) 10431
RRIDAB_2882754
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.

Protocols

Product Specific Protocols
WB protocol for Tim23 antibody 67535-1-IgDownload protocol
IHC protocol for Tim23 antibody 67535-1-IgDownload protocol
IF protocol for Tim23 antibody 67535-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

The nucleoprotein of influenza A virus inhibits the innate immune response by inducing mitophagy

Authors - Bo Zhang
mouseWB, IF

Autophagy

Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival.

Authors - Yinjuan Song
mouseWB

Mol Med

4-Octyl itaconate attenuates LPS-induced acute kidney injury by activating Nrf2 and inhibiting STAT3 signaling

Authors - Lujun Xu
humanWB

Zool Res

Choline dehydrogenase interacts with SQSTM1 to activate mitophagy and promote coelomocyte survival in Apostichopus japonicus following Vibrio splendidus infection

Authors - Lian-Lian Sun
mouseWB

J Biol Chem

NOX4-TIM23 interaction regulates NOX4 mitochondrial import and metabolic reprogramming

Authors - Jyotsana Pandey