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CoraLite® Plus 488-conjugated Tim23 Monoclonal antibody

Tim23 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3H10F5

Cat no : CL488-67535

Synonyms

PRO1197, TIM23, TIMM23

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Tim23 Monoclonal ant (CL488-67535)

    IF Staining of HepG2 using CL488-67535

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Tim23 antibody (CL488-67535, Clone: 3H10F5 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67535 targets Tim23 in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Tim23 fusion protein Ag27069
Full Name translocase of inner mitochondrial membrane 23 homolog (yeast)
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC062707
Gene Symbol TIMM23
Gene ID (NCBI) 10431
RRIDAB_2919495
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Tim23 antibody CL488-67535Download protocol
Standard Protocols
Click here to view our Standard Protocols