Validation Data Gallery
|Positive WB detected in
|LNCaP cells, 4T1 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurket cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
|Positive IHC detected in
|mouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:1000-1:4000
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
68166-1-Ig targets U2AF2/U2AF65 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.
|Human, rat, mouse
|Host / Isotype
|Mouse / IgG2a
|U2AF2/U2AF65 fusion protein Ag8070
|U2 small nuclear RNA auxiliary factor 2
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
U2AF2, also named as U2 auxiliary factor 65 kDa subunit, is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.
|Product Specific Protocols
|WB protocol for U2AF2/U2AF65 antibody 68166-1-Ig
|IHC protocol for U2AF2/U2AF65 antibody 68166-1-Ig
|IF protocol for U2AF2/U2AF65 antibody 68166-1-Ig
|FC protocol for U2AF2/U2AF65 antibody 68166-1-Ig
|Click here to view our Standard Protocols