U2AF2/U2AF65 Monoclonal antibody, PBS Only

U2AF2/U2AF65 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B7E7

Cat no : 68166-1-PBS

Synonyms

hU2AF(65), hU2AF65, U2AF2, U2AF65

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Validation Data Gallery

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  WB analysis using 68166-1-Ig (same clone as 68166-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 68166-1-Ig (same clone as 68166-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68166-1-Ig (same clone as 68166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68166-1-Ig (same clone as 68166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 68166-1-Ig (same clone as 68166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human U2AF2 (68166-1-Ig, Clone:1B7E7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68166-1-PBS targets U2AF2/U2AF65 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: U2AF2/U2AF65 fusion protein Ag8070
• Full Name: U2 small nuclear RNA auxiliary factor 2
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC008740
• Gene Symbol: U2AF2
• Gene ID (NCBI): 11338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

U2AF2, also named as U2 auxiliary factor 65 kDa subunit, is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.