UBE2S Polyclonal antibody

• Featured Product
• KD/KO Validated

UBE2S Polyclonal Antibody for WB, IP, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 14115-1-AP

Synonyms

E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S

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Validation Data Gallery

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  WB analysis using 14115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14115-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 14115-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14115-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 14115-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14115-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

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  WB analysis of HEK-293T using 14115-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

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  WB analysis of MCF-7 using 14115-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  IP experiment of A2780 using 14115-1-AP

  IP result of anti-UBE2S (IP:14115-1-AP, 4ug; Detection:14115-1-AP 1:1000) with A2780 cells lysate 960ug.

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  IHC staining of human liver cancer using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14115-1-AP (UBE2S antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14115-1-AP (UBE2S antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 14115-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14115-1-AP (UBE2S antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Jurkat using 14115-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug UBE2S antibody (14115-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293T cells, mouse liver tissue, A2780 cells, human liver tissue, MDA-MB-453s cells, HeLa cells, MOLT-4 cells, PC-3 cells, HEK-293 cells
• Positive IP detected in: A2780 cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human skin tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• CoIP: See 3 publications below

Product Information

14115-1-AP targets UBE2S in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE2S fusion protein Ag5264
• Full Name: ubiquitin-conjugating enzyme E2S
• Calculated Molecular Weight: 222 aa, 24 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC066948
• Gene Symbol: UBE2S
• Gene ID (NCBI): 27338
• RRID: AB_2256809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

Publications

Species	Application	Title
human	WB	EMBO J; Weakened APC/C activity at mitotic exit drives cancer vulnerability to KIF18A inhibition; Authors - Colin R Gliech ; KD Validated
mouse	WB	Protein Cell; BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding.; Authors - Mingzhu Wang ; KD Validated
human	WB,IHC	Int J Biol Sci; UBE2S as a novel ubiquitinated regulator of p16 and β-catenin to promote bone metastasis of prostate cancer.; Authors - Shengmeng Peng ; KD Validated
human	WB	Int J Biol Sci; The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.; Authors - Wei Xu
human	WB,IHC	J Ovarian Res; UBE2S promotes the progression and Olaparib resistance of ovarian cancer through Wnt/β-catenin signaling pathway.; Authors - Wenjing Hu
human	WB,IHC	Biochem Biophys Res Commun; UBE2S associated with OSCC proliferation by promotion of P21 degradation via the ubiquitin-proteasome system.; Authors - Shusaku Yoshimura ; KD Validated