Tested Applications
| Positive WB detected in | MCF-7 cells, HEK-293 cells, HEK-293T cells, MOLT-4 cells, PC-3 cells, HeLa cells |
| Positive IP detected in | A2780 cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human skin tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14115-1-AP targets UBE2S in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5264 Product name: Recombinant human UBE2S protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-222 aa of BC066948 Sequence: MNSNVENLPPHIIRLVYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHLNVGANGEICVNVLKRDWTAELGIRHVLLTIRCLLIHPNPESALNEEAGRLLLENSEEYAARARLLTEIHGGAGGPSGRAEAGRALASGTAASSTDPGAPGGLGGAEGPMAKKHAGERDKKLAAKKKTDKKRALRRL Predict reactive species |
| Full Name | ubiquitin-conjugating enzyme E2S |
| Calculated Molecular Weight | 222 aa, 24 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC066948 |
| Gene Symbol | UBE2S |
| Gene ID (NCBI) | 27338 |
| RRID | AB_2256809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16763 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UBE2S antibody 14115-1-AP | Download protocol |
| IP protocol for UBE2S antibody 14115-1-AP | Download protocol |
| WB protocol for UBE2S antibody 14115-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UBE2S antibody (Cat# 14115-1-AP) has 26 citations published in journals including Cell Reports, EMBO Journal, Protein & Cell, Cell Death & Disease, International Journal of Biological Sciences, Journal of Translational Medicine, Human Molecular Genetics, Autophagy, and Molecular Carcinogenesis. Research fields span oncology (hepatocellular, prostate, ovarian, glioblastoma, lung, colorectal, renal, and esophageal cancers), ubiquitination biology, metabolic reprogramming, viral replication, and cell cycle regulation.
| Species | Application | Title |
|---|---|---|
Clin Mol Hepatol UBE2S promotes glycolysis in hepatocellular carcinoma by enhancing E3 enzyme-independent polyubiquitination of VHL | ||
Protein Cell BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding.
| ||
Cell Death Dis UBE2S interacting with TRIM21 mediates the K11-linked ubiquitination of LPP to promote the lymphatic metastasis of bladder cancer
| ||
Int J Biol Sci The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer. | ||
Int J Biol Sci UBE2S as a novel ubiquitinated regulator of p16 and β-catenin to promote bone metastasis of prostate cancer.
| ||
J Transl Med UBE2S promotes small cell lung cancer transformation and progression via monitoring metabolic remodeling and cell cycle. |
