CoraLite® Plus 488-conjugated USP13 Monoclonal antibody

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  IF Staining of HepG2 using CL488-66176

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated USP13 antibody (CL488-66176, Clone: 6E8C10 ) at dilution of 1:100.

• 

  IF Staining of HepG2 using CL488-66176

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 USP13 antibody (CL488-66176, Clone: 6E8C10 ) at dilution of 1:200.

• Featured Product
• KD/KO Validated

USP13 Monoclonal Antibody for IF/ICC

Cat No. CL488-66176

Clone No.6E8C10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

Ubiquitin carboxyl-terminal hydrolase 13, ISOT-3, ISOT3, Isopeptidase T-3, EC:3.4.19.12

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66176 targets USP13 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10506

  Product name: Recombinant human USP13 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 515-863 aa of BC016146

  Sequence: IAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPS

• Full Name: ubiquitin specific peptidase 13 (isopeptidase T-3)
• Calculated Molecular Weight: 863 aa, 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC016146
• Gene Symbol: USP13
• Gene ID (NCBI): 8975
• RRID: AB_2934450
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q92995
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.