Filter: AllWBIHC

WB analysis using 11444-1-AP (same clone as 11444-1-PBS)
Untreated or TNF-a-treated HUVEC cells were subjected to SDS PAGE followed by western blot with 11444-1-AP (VCAM-1/CD106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11444-1-AP (VCAM-1/CD106 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11444-1-AP (VCAM-1/CD106 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 11444-1-AP (VCAM-1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 11444-1-AP (same clone as 11444-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11444-1-AP (VCAM-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11444-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

VCAM-1/CD106 Polyclonal antibody, PBS Only

VCAM-1/CD106 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11444-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

VCAM1, VCAM-1, CD106, INCAM 100, INCAM-100

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

11444-1-PBS targets VCAM-1/CD106 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1993 Product name: Recombinant human VCAM-1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 341-739 aa of BC017276 Sequence: ESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPELLVLYFASSLIIPAIGMIIYFARKANMKGSYSLVEAQKSKV Predict reactive species
Full Name	vascular cell adhesion molecule 1
Calculated Molecular Weight	739 aa, 81 kDa
Observed Molecular Weight	110 kDa
GenBank Accession Number	BC017276
Gene Symbol	VCAM-1
Gene ID (NCBI)	7412
RRID	AB_2214232
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P19320
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).