VGF Polyclonal antibody, PBS Only

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• VGF Polyclonal antibody, PBS Only (26781-1-PBS)

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  WB analysis using 26781-1-AP (same clone as 26781-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26781-1-AP (VGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26781-1-PBS in a different storage buffer formulation.

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  IP experiment of U2OS using 26781-1-AP (same clone as 26781-1-PBS)

  U2OS lysates prepared and IP of VGF performed using 1.0 μg of 26781-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 26781-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 26781-1-AP (same clone as 26781-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26781-1-AP (VGF Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 26781-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 26781-1-AP (same clone as 26781-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26781-1-AP (VGF Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 26781-1-PBS in a different storage buffer formulation.

VGF Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 26781-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

SCG7, Neurosecretory protein VGF, Neuro-Endocrine Specific Protein VGF, Neuroendocrine regulatory peptide-2, Neuroendocrine regulatory peptide-1

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Product Information

26781-1-PBS targets VGF in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24820

  Product name: Recombinant human VGF protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 81-280 aa of BC063835

  Sequence: ALAAVLLQALDRPASPPAPSGSQQGPEEEAAEALLTETVRSQTHSLPAPESPEPAAPPRPQTPENGPEASDPSEELEALASLLQELRDFSPSSAKRQQETAAAETETRTHTLTRVNLESPGPERVWRASWGEFQARVPERAPLPPPAPSQFQARMPDSGPLPETHKFGEGVSSPKTHLGEALAPLSKAYQGVAAPFPKAR

• Full Name: VGF nerve growth factor inducible
• Calculated Molecular Weight: 615 aa, 67 kDa
• Observed Molecular Weight: 67 kDa, 90-100 kDa
• GenBank Accession Number: BC063835
• Gene Symbol: VGF
• Gene ID (NCBI): 7425
• RRID: AB_2880632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15240
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

VGF encodes a neuropeptide precursor with specific expression in a subpopulation of neuroendocrine cells, and it is upregulated by nerve growth factor. The expression of VGF is developmentally regulated in different areas of the brain (PubMed: 18406753). Exercise upregulated expression of VGF in mouse hippocampus, and a synthetic Vgf-derived peptide produced an antidepressant response in mice (PubMed: 18059283). VGF can be detected the precursor form of 90-100 kDa before proteolytic cleavage (PMID:24934694, PMID:10433233).