|Positive WB detected in||human brain tissue, human heart tissue, HEK-293 cells, human kidney tissue, mouse pancreas tissue, mouse brain tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19140-1-AP is VPS11 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VPS11 fusion protein Ag6227|
|Full Name||vacuolar protein sorting 11 homolog (S. cerevisiae)|
|Calculated molecular weight||108 kDa|
|Observed molecular weight||108-112 kDa|
|GenBank accession number||BC065563|
|Gene ID (NCBI)||55823|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. VPS11 is the human homolog of the yeast class C Vps11 protein, a subunit of HOPS (homotypic fusion and protein transport) complex. Mammalian Vps11 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
|Product Specific Protocols|
|WB protocol for VPS11 antibody 19140-1-AP||Download protocol|
|IF protocol for VPS11 antibody 19140-1-AP||Download protocol|
|IP protocol for VPS11 antibody 19140-1-AP||Download protocol|
|FC protocol for VPS11 antibody 19140-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Inherit Metab Dis
The second report of a new hypomyelinating disease due to a defect in the VPS11 gene discloses a massive lysosomal involvement.
VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.
J Cell Sci
The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome.
J Biol Chem
Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity.
J Mol Biol
Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.
J Cell Sci
Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.