VPS13A Polyclonal antibody, PBS Only

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• VPS13A Polyclonal antibody, PBS Only (28618-1-PBS)

• 

  WB analysis using 28618-1-AP (same clone as 28618-1-PBS)

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 28618-1-AP (VPS13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28618-1-AP (same clone as 28618-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 28618-1-AP (VPS13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 28618-1-AP (same clone as 28618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28618-1-AP (same clone as 28618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 28618-1-AP (same clone as 28618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 28618-1-AP (same clone as 28618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

VPS13A Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28618-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

CHAC, Intermembrane lipid transfer protein VPS13A, Vacuolar protein sorting-associated protein 13A

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Product Information

28618-1-PBS targets VPS13A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29532

  Product name: Recombinant human VPS13A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2452-2595 aa of NM_033305

  Sequence: PVGSRRLKWRCRKSHGEVTQKDDMMMPIDLGEKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAEQEIAVALQDVGISLVNNYTKQEVAYIGITSSDVVWETKPKKKARWKPMSVKHTEKLEREFKEYTESSPSEDKVI

• Full Name: vacuolar protein sorting 13 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 360 kDa
• Observed Molecular Weight: 360 kDa
• GenBank Accession Number: NM_033305
• Gene Symbol: VPS13A
• Gene ID (NCBI): 23230
• RRID: AB_2918183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96RL7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).