Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue, mouse ovary tissue, rat epididymis tissue, rat ovary tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IF | See 4 publications below |
Product Information
22587-1-AP targets WBP2NL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18347 Product name: Recombinant human WBP2NL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-309 aa of BC022546 Sequence: MAVNQSHTENRRGALIPNGESLLKRSPNVELSFPQRSEGSNVFSGRKTGTLFLTSYRVIFITSCSISDPMLSFMMPFDLMTNLTVEQPVFAANFIKGTIQAAPYGGWEGQATFKLVFRNGGAIEFAQLMVKAASAAARGFPLRTLNDWFSSMGIYVITGEGNMCTPQMPCSVIVYGAPPAGYGAPPPGYGAPPAGYGAQPVGNEGPPVGYRASPVRYGAPPLGYGAPPAGYGAPPLGYGAPPLGYGTPPLGYGAPPLGYGAPPAGNEGPPAGYRASPAGSGARPHESTAAQAPENEASLPSASSSQVHS Predict reactive species |
| Full Name | WBP2 N-terminal like |
| Calculated Molecular Weight | 309 aa, 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC022546 |
| Gene Symbol | WBP2NL |
| Gene ID (NCBI) | 164684 |
| RRID | AB_2879128 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ICG8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WBP2NL, also known as PAWP, is a protein found in the post-acrosomal region of mammalian spermatozoa. It was initially proposed as a sperm-borne oocyte-activating factor (SOAF) that triggers calcium oscillations essential for oocyte activation and embryo development. However, recent studies have shown mixed results regarding its role in fertilization. While some investigations suggest that PAWP can induce oocyte activation, others indicate that it may not be sufficient to compensate for the absence of other critical factors like PLCZ1. Additionally, altered WBP2NL expression has been observed in breast cancer tissues, hinting at its potential involvement in oncogenic regulation. (PMID: 24907910, PMID: 25417742, PMID: 28663133)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WBP2NL antibody 22587-1-AP | Download protocol |
| IHC protocol for WBP2NL antibody 22587-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW binding protein PAWP. | ||
Int J Mol Sci Comprehensive Flow-Cytometric Quality Assessment of Ram Sperm Intended for Gene Banking Using Standard and Novel Fertility Biomarkers. | ||
Development PLCζ is the physiological trigger of the Ca(2+) oscillations that induce embryogenesis in mammals but offspring can be conceived in its absence. | ||
Mol Hum Reprod Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation. | ||
Mol Hum Reprod Antigen unmasking enhances visualization efficacy of the oocyte activation factor, phospholipase C zeta, in mammalian sperm. |
