WDR81 Polyclonal antibody, PBS Only

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• WDR81 Polyclonal antibody, PBS Only (24874-1-PBS)

• 

  WB analysis using 24874-1-AP (same clone as 24874-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24874-1-AP (WDR81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 24874-1-AP (same clone as 24874-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24874-1-AP (WDR81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 24874-1-AP (same clone as 24874-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24874-1-AP (WDR81 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 24874-1-AP (same clone as 24874-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24874-1-AP (WDR81 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 24874-1-AP (same clone as 24874-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using WDR81 antibody (24874-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 24874-1-AP (same clone as 24874-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WDR81 antibody (24874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

WDR81 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 24874-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

WD repeat-containing protein 81

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Product Information

24874-1-PBS targets WDR81 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21666

  Product name: Recombinant human WDR81 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 323-692 aa of BC114568

  Sequence: NDSRPENPGPLGPISGVGGGGLGSGSDDNALKQELPRSVHGLSGNWLAYWQYEIGVSQQDAHFHFHQIRLQSFPGHSGAVKCVAPLSSEDFFLSGSKDRTVRLWPLYNYGDGTSETAPRLVYTQHRKSVFFVGQLEAPQHVVSCDGAVHVWDPFTGKTLRTVEPLDSRVPLTAVAVMPAPHTSITMASSDSTLRFVDCRKPGLQHEFRLGGGLNPGLVRALAISPSGRSVVAGFSSGFMVLLDTRTGLVLRGWPAHEGDILQIKAVEGSVLVSSSSDHSLTVWKELEQKPTHHYKSASDPIHTFDLYGSEVVTGTVSNKIGVCSLLEPPSQATTKLSSENFRGTLTSLALLPTKRHLLLGSDNGVIRLLA

• Full Name: WD repeat domain 81
• Calculated Molecular Weight: 1941 aa, 212 kDa
• Observed Molecular Weight: 74-80 kDa, 250 kDa
• GenBank Accession Number: BC114568
• Gene Symbol: WDR81
• Gene ID (NCBI): 124997
• RRID: AB_2879771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q562E7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

WD repeat-containing protein 81 (WDR81) is a 1941 amino acid protein, which contains one BEACH domain and five WD repeats. WDR81 is widely expressed in the brain. WDR81 has an association with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 2. WDR81 exists as six isoforms.