WIBG Recombinant antibody

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  WB analysis using 86064-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86064-1-RR (WIBG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86064-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86064-1-RR (WIBG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

WIBG Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86064-1-RR

Clone No.242800G10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Partner of Y14 and mago, Protein wibg homolog, PYM, PYM homolog 1 exon junction complex-associated factor, PYM1

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, SH-SY5Y cells, HepG2 cells, U-251 cells, HL-60 cells, mouse thymus tissue, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86064-1-RR targets WIBG in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: WIBG fusion protein Ag6276
• Full Name: within bgcn homolog (Drosophila)
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23-27 kDa
• GenBank Accession Number: BC006135
• Gene Symbol: WIBG
• Gene ID (NCBI): 84305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BRP8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein WIBG homolog, also known as PYM1 (Partner of Y14 and mago), is a cytoplasmic RNA-binding protein, that can interact with the ribosome and EJC, may act as an EJC destabilizing factor, preventing EJC accumulation at positions away from exon-exon junctions (non-canonical position).