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ZBTB20 Polyclonal antibody, PBS Only (23987-1-PBS)

Filter: AllWBIHCIF-P

WB analysis using 23987-1-AP (same clone as 23987-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.
WB analysis of L02 using 23987-1-AP (same clone as 23987-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 23987-1-AP (same clone as 23987-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 23987-1-AP (same clone as 23987-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 23987-1-AP (same clone as 23987-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 23987-1-AP (same clone as 23987-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 23987-1-AP (same clone as 23987-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.
WB analysis of RAW 264.7 using 23987-1-AP (same clone as 23987-1-PBS)
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 23987-1-AP (same clone as 23987-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 23987-1-AP (same clone as 23987-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 23987-1-AP (same clone as 23987-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.
IF Staining of mouse liver using 23987-1-AP (same clone as 23987-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.
IF Staining of mouse liver using 23987-1-AP (same clone as 23987-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

ZBTB20 Polyclonal antibody, PBS Only

Name: ZBTB20 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 23987-1-PBS

ZBTB20 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 23987-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Dendritic-derived BTB/POZ zinc finger protein, DPZF, Zinc finger and BTB domain-containing protein 20, Zinc finger protein 288, ZNF288

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

23987-1-PBS targets ZBTB20 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21140 Product name: Recombinant human ZBTB20 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 313-668 aa of BC029041 Sequence: EPDSVEQQFGPGAARDSQAEPTQPEQAAEAPAEGGPQTNQLETGASSPERSNEVEMDSTVITVSNSSDKSVLQQPSVNTSIGQPLPSTQLYLRQTETLTSNLRMPLTLTSNTQVIGTAGNTYLPALFTTQPAGSGPKPFLFSLPQPLAGQQTQFVTVFQPGLSTFTAQLPAPQPLASSAGHSTASGQGEKKPYECTLCNKTFTAKQNYVKHMFVHTGEKPHQCSICWRSFSLKDYLIKHMVTHTGVRAYQCSICNKRFTQKSSLNVHMRLHRGEKSYECYICKKKFSHKTLLERHVALHSASNGTPPAGTPPGARAGPPGVVACTEGTTYVCSVCPAKFDQIEQFNDHMRMHVSDG Predict reactive species
Full Name	zinc finger and BTB domain containing 20
Calculated Molecular Weight	668 aa, 73 kDa
Observed Molecular Weight	73 kDa
GenBank Accession Number	BC029041
Gene Symbol	ZBTB20
Gene ID (NCBI)	26137
RRID	AB_2879389
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q9HC78
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZBTB20 (zinc finger and BTB domain containing 20), also known as HOF, DPZF, ODA-8S or ZNF288, is a 741 amino acid protein that localizes to the nucleus and contains one BTB (POZ) domain and five C2H2-type zinc fingers. Expressed in thymus, spleen, lymph node and fetal liver, ZBTB20 exists as either a monomer or a homodimer that is thought to function as a transcription factor, playing a role in hematopoiesis, oncogenesis and immune responses. Multiple isoforms of ZBTB20 exist due to alternative splicing events.