ZW10 Recombinant monoclonal antibody

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• ZW10 Recombinant monoclonal antibody (86726-1-RR)

• 

  WB analysis using 86726-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86726-1-RR (ZW10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 86726-1-RR

  WB result of ZW10 antibody (86726-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZW10 transfected HeLa cells.

• 

  IF Staining of Jurkat using 86726-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ZW10 antibody (86726-1-RR, Clone: 251397A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

ZW10 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86726-1-RR

Clone No.251397A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

HZW10

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, Ramos cells, K-562 cells
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86726-1-RR targets ZW10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19138

  Product name: Recombinant human ZW10 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 318-672 aa of BC128264

  Sequence: NEKTSTVPLAEMLGDMIWEDLSECLIKNCLVYSIPTNSSKLQQYEEIIQSTEEFENALKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARNLMTSEIHNTVKIIPDSKINVPELPTPDEDNKLEVQKVSNTQYHEVMNLEPENTLDQHSFSLPTCRISESVKKLMELAYQTLLEATTSSDQCAVQLFYSVRNIFHLFHDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRLRLAPILCDGTATFVDLVPGFRRLGTECFLAQMRAQKGELLERLSSARNFSNMDDEENYSAASKAVRQVLHQLKRLGIVWQDVLPVNIYCKAMGTLLNTAISEVIGKITALE

• Full Name: ZW10, kinetochore associated, homolog (Drosophila)
• Calculated Molecular Weight: 779 aa, 89 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC128264
• Gene Symbol: ZW10
• Gene ID (NCBI): 9183
• RRID: AB_3745082
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43264
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZW10 is the human homolog of the Drosophila melanogaster Zw10 protein. ZW10 is localized to kinetochores and kinetochore-associated microtubules and is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis(PMID: 17102640). ZW10 proteins in Drosophila and HeLa cells display a similar and intriguing cell cycle-dependent intracellular distribution. ZW10 protein first becomes localized to the kinetochore at prometaphase, but then appears to move onto the kinetochore microtubules of the spindle at metaphase, and then back to the kinetochore at anaphase(PMID: 9700164).