ChromoTek anti IFNy recombinant VHH, FITC Plus

Validation Data Gallery

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  HeLa cells transiently transfected with human IFNg were immunostained with anti IFNy recombinant VHH, FITC Plus (FITC-IFNg, green). Nuclei are in blue. Cells were treated with Brefeldin A and Monensin for 6 hours.

FITC-IFNg is a recombinant FITC Plus conjugated anti IFNg Nanobody (VHH) that targets IL6 in IF and FC applications, showing reactivity with human samples.

Cat No. FITC-IFNg

Host/Type

Alpaca VHH

Reactivity

Human

Applications

IF, FC

Conjugate

FITC Plus

Synonyms

IFNg VHH, IFNg single domain antibody (sdAb), Interferon Gamma, Immune interferon, Interferon

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Product Information

FITC-IFNg is a recombinant FITC Plus conjugated anti IFNg Nanobody (VHH) that targets IL6 in IF and FC applications, showing reactivity with human samples.

• Applications: IF, FC
• Reactivity: Human
• Conjugate: FITC Plus
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity: Picomolar range, below the assay limit (biolayer interferometry)
• Molecular Weight: 14.8 kDa
• Form: Liquid
• Excitation/Emission Maxima Wavelengths: 500 nm / 520 nm
• RRID: AB_3665970
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide
• Storage Condition: Store at -20°C
• Shipping: blue ice
• Background: IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB).

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