ChromoTek anti-Mouse IgG1 Magnetic VHH Agarose for Immunoprecipitation

Validation Data Gallery

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  Co-IP of MCM complex via pulldown of MCM6 using 5 µg of monoclonal anti-MCM6 IgG1 (Proteintech: 67989-1-Ig) and anti-mouse IgG1 magnetic VHH agarose (mIG1ma). 5 µg of each IgG was spiked into HEK293T cell lysate derived from 1x10^7 cells. All subunits of the 600 kDa hetero-hexameric complex are successfully precipitated, as shown in a Coomassie blue stained SDS-gel and by Western blot analysis using subunit specific antibodies. Apparent molecular weights are provided. For input (IN) and flowthrough (FT) fractions, 1% was loaded, respectively. For bound (B) fraction and bound fraction of isotype control antibody (BISO PTG: 66360-1-Ig), 25% was loaded. Product codes for Proteintech antibodies are provided in parentheses. Compared to Protein A magnetic agarose, mIG1ma shows a superior precipitation of the MCM complex.

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  IP of PCNA by anti-mouse IgG1 magnetic VHH agarose (mIG1ma) using the PCNA Monoclonal antibody (PTG: 60097-1-Ig). As control, a IgG1 isotype control antibody (Proteintech: 66360-1-Ig) was used (BISO). 5 µg of each IgG was spiked into HEK293T cell lysate derived from 0.5x10^7 cells. 1% of input (IN) and flow through (FT) and 25% of bound (B) fraction was loaded onto an SDS-PAGE gel. For Western blot analysis PCNA was detected using a polyclonal rabbit IgG (Proteintech: 10205-2-AP) (1:5000) and an HRP-labeled goat anti-rabbit secondary (Proteintech: RGAR001). Compared to Protein A and G magnetic beads, mIG1ma shows a superior precipitation and allows for a cleaner detection of PCNA.

anti-Mouse IgG1 IP Beads is an affinity resin for IP of Mouse IgG1. It consists of rabbit specific VHHs (Nanobodies) coupled to Magnetic Agarose beads.

Cat No. mIG1ma

Reactivity

Mouse IgG1

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Magnetic Agarose

Synonyms

Mouse IP, IgG IP, IgG Trap, Secondary VHH, VHH, Nanobody

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Product Information

anti-Mouse IgG1 IP Beads is an affinity resin for IP of Mouse IgG1. It consists of rabbit specific VHHs (Nanobodies) coupled to Magnetic Agarose beads.

• Description: Ready to use VHH coupled Magnetic Agarose Beads for Immunoprecipitation of Mouse IgG1. Add bulletpoints from below here:;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • Stable in harsh buffer conditions;

  • Suitable for downstream mass spec analysis;

  • High Specificity;

  • Low background

• Applications: IP, Co-IP
• Binding capacity: /
• Conjugate: Magnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
• Elution buffer: SDS Sample Buffer
• Wash buffer compatibility: /
• Type: Nanobody
• Class: Recombinant - Animal free production
• Host: Alpaca
• Affinity (KD): /
• Immunogen: Mouse IgG1
• RRID: AB_3697093
• Storage Buffer: 20% Ethanol
• Storage Condition: +4°C / do not freeze!
• Shipping: Ambient Temp
• Size: 1 ml; 2 ml; 5 ml

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