ChromoTek anti-Mouse IgG2b Magnetic VHH Agarose for Immunoprecipitation

Validation Data Gallery

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  Co-IP of BAX and BAK by anti-mouse IgG2b VHH agarose (mIG2bma) using the BAX Monoclonal antibody 4G5E8 (Proteintech: 60267-1-Ig). As control a IgG2b isotype control antibody (Proteintech: 66360-3-Ig) was used (BISO). 5 µg of each IgG was spiked into HEK293T cell lysate derived from 0.5x10^7 cells. 1% of input (IN) and flow through (FT) and 25% of bound (B) fraction was loaded onto an SDS-PAGE gel. For Western blot analysis BAX was detected using a polyclonal rabbit IgG (Proteintech: 50599-2-Ig) (1:2000) labeled using a FlexAble HRP (Proteintech: KFA065). The presence of BAK co-precipitated with BAX was confirmed using a polyclonal rabbit IgG (PTG 29552-1-AP) (1:2000).

anti-Mouse IgG2b IP Beads is an affinity resin for IP of Mouse IgG2b. It consists of rabbit specific VHHs (Nanobodies) coupled to Magnetic Agarose beads.

Cat No. mIG2bma

Reactivity

Mouse IgG2b

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Magnetic Agarose

Synonyms

Mouse IP, IgG IP, IgG Trap, Secondary VHH, VHH, Nanobody

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Product Information

anti-Mouse IgG2b IP Beads is an affinity resin for IP of Mouse IgG2b. It consists of rabbit specific VHHs (Nanobodies) coupled to Magnetic Agarose beads.

• Description: Ready to use VHH coupled Magnetic Agarose Beads for Immunoprecipitation of Mouse IgG2b. Add bulletpoints from below here:;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • Stable in harsh buffer conditions;

  • Suitable for downstream mass spec analysis;

  • High Specificity;

  • Low background

• Applications: IP, Co-IP
• Binding capacity: /
• Conjugate: Magnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
• Elution buffer: SDS Sample Buffer
• Wash buffer compatibility: /
• Type: Nanobody
• Class: Recombinant - Animal free production
• Host: Alpaca
• Affinity (KD): /
• Immunogen: Mouse IgG2b
• RRID: AB_3697097
• Storage Buffer: 20% Ethanol
• Storage Condition: +4°C / do not freeze!
• Shipping: Ambient Temp
• Size: 1 ml; 2 ml; 5 ml

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